Verification of total cholesterol testing method using an automated chemistry analyzer based on accuracy, precision, and linearity parameters at Cimanggu I Public Health Center
Abstract
Background: Checking total cholesterol is one of the most common lab tests to spot and keep track of high cholesterol. To get reliable results, the tests need to be accurate, precise, and consistent. Objectives: This study looked at how well the Rayto Chemray 240 automated chemistry analyzer measures total cholesterol, focusing on accuracy, precision, and consistency Materials and Methods: We did an experimental lab study at the Clinical Laboratory of Cimanggu I Public Health Center. To check precision, we measured quality control serum ten times in one run and calculated the coefficient of variation (CV). Accuracy was checked by measuring quality control serum over twenty days and reporting recovery percentage, bias, and total error. We checked linearity using a range of cholesterol standard concentrations (0–100%) and analyzed it with linear regression Results: The precision test gave an average value of 144.67 mg/dL, a standard deviation of 3.07, and a CV of 2.12%. Accuracy results showed a recovery of 103.07%, bias of 3.07%, and total error of 9.61%, which is under the allowed limit of 10%. The linearity test produced the regression equation y = 1.95x + 19.85 with a correlation coefficient (r) of 0.999 and R² of 0.998. Conclusions: The total cholesterol test using the Rayto Chemray 240 analyzer met the standards for precision, accuracy, and linearity, so it’s good to use for regular lab tests.
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