Effectiveness of a modified double-trap mosquito device with rice straw infusion attractant in increasing ovitrap and gravidtrap indices

Main Article Content

Alsya Latisa
Rosa Nur Munawaroh
Sri Nuraeni
Mela Nurfadila
Rochmanah Suhartati
Rudy Hidana
Tanendri Arrizqiyani
Dewi Peti
Dina Ferdiani

Abstract

The modified double-trap mosquito device combines ovitrap and gravidtrap functions in a single container to capture mosquito eggs and gravid adults, with application for Aedes aegypti surveillance in dengue-endemic households. This study assessed whether adding a 0.83% rice straw infusion attractant improved the ovitrap index (OI), gravidtrap index (GI), and trap catches compared with a control device. An experiment was conducted in 100 houses in Kampung Sindangsuka, Gunung Gede Village, Kawalu District, Tasikmalaya City, from 14 to 27 April 2024. Each house received one modified device and one control device, yielding 100 per group. Traps were placed in humid, low-light indoor locations and monitored for two weeks. Mosquito eggs and adults were counted, although specimens were not identified to species level. OI and GI were calculated as percentages of positive traps, while egg and adult counts were compared using the Mann–Whitney U test after normality assessment. The modified device achieved higher OI and GI values than the control, with an OI of 62% versus 12% and a GI of 52% versus 23%. It collected 8,002 eggs, averaging 80.02 eggs per device and representing 91% of all eggs, and 94 adult mosquitoes, averaging 0.94 per device and representing 72% of all adults. The control collected 746 eggs and 36 adults. Differences in egg and adult catches were significant (p < 0.001). These findings indicate that 0.83% rice straw infusion enhances trap attractiveness and detection capacity, supporting its use as a low-cost tool for Aedes surveillance and community-based dengue vector control.

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How to Cite
Latisa, A., Munawaroh, R. N., Nuraeni, S., Nurfadila, M., Suhartati, R., Hidana, R., … Ferdiani, D. (2026). Effectiveness of a modified double-trap mosquito device with rice straw infusion attractant in increasing ovitrap and gravidtrap indices. Journal of Indonesian Medical Laboratory and Science (JoIMedLabS), 7(2), 25–36. https://doi.org/10.53699/joimedlabs.v7i2.314
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Artikel
Author Biographies

Alsya Latisa, Universitas Bakti Tunas Husada

Program Studi D3 Analis Kesehatan, Fakultas Ilmu Kesehatan, Universitas Bakti Tunas Husada, Tasikmalaya, Indonesia

Rosa Nur Munawaroh, Universitas Bakti Tunas Husada

Program Studi D3 Analis Kesehatan, Fakultas Ilmu Kesehatan, Universitas Bakti Tunas Husada, Tasikmalaya, Indonesia

Sri Nuraeni, Universitas Bakti Tunas Husada

Program Studi D3 Analis Kesehatan, Fakultas Ilmu Kesehatan, Universitas Bakti Tunas Husada, Tasikmalaya, Indonesia

Mela Nurfadila, Universitas Bakti Tunas Husada

Program Studi D3 Analis Kesehatan, Fakultas Ilmu Kesehatan, Universitas Bakti Tunas Husada, Tasikmalaya, Indonesia

Rochmanah Suhartati, Universitas Bakti Tunas Husada

Program Studi D3 Analis Kesehatan, Fakultas Ilmu Kesehatan, Universitas Bakti Tunas Husada, Tasikmalaya, Indonesia

Rudy Hidana, Universitas Bakti Tunas Husada

Program Studi D3 Analis Kesehatan, Fakultas Ilmu Kesehatan, Universitas Bakti Tunas Husada, Tasikmalaya, Indonesia

Tanendri Arrizqiyani, Universitas Bakti Tunas Husada

Program Studi D3 Analis Kesehatan, Fakultas Ilmu Kesehatan, Universitas Bakti Tunas Husada, Tasikmalaya, Indonesia

Dewi Peti, Universitas Bakti Tunas Husada

Program Studi D3 Analis Kesehatan, Fakultas Ilmu Kesehatan, Universitas Bakti Tunas Husada, Tasikmalaya, Indonesia

Dina Ferdiani, Universitas Bakti Tunas Husada

Program Studi D3 Analis Kesehatan, Fakultas Ilmu Kesehatan, Universitas Bakti Tunas Husada, Tasikmalaya, Indonesia

References

Adams, L. E., Wong, J. M., & Paz-Bailey, G. (2024). Dengue. JAMA, 332(24), 2109-2110. https://doi.org/10.1001/jama.2024.21094

Agustina, E., & Kartini. (2018). Jenis Wadah Tempat Perindukan Larva Nyamuk Aedes di Gapong Binan Akademi Kesehatan Lingkungan. Prosiding Seminar Nasional Biotik 2018, 6(1), 600–606. http://dx.doi.org/10.22373/pbio.v6i1.4302

Coutinho-Abreu, I. V., Riffell, J. A., & Akbari, O. S. (2022). Human attractive cues and mosquito host-seeking behavior. Trends in Parasitology, 38(3), 246-264. https://doi.org/10.1016/j.pt.2021.09.012

Dinkes Jabar. (2024). Jumlah Kasus Penyakit Demam Berdarah Dengue (DBD) Berdasarkan Kabupaten/Kota di Jawa Barat. https://opendata.jabarprov.go.id/id/dataset/jumlah-kasus-penyakit-demam-berdarah-dengue-dbd-berdasarkan-kabupatenkota-di-jawa-barat

Dinkes Provinsi NTB. (2017). Obat Pembunuh Jentik Nyamuk (ABATE). https://dinkes.ntbprov.go.id/artikel/obat-pembunuh-jentik-nyamuk-abate/

Food and Environment Hygienie Department (FEHD). (2024). Vector-borne diseases. https://www.fehd.gov.hk/english/pestcontrol/dengue_fever/index.html

Hasanah, H. U., Sukamto, D. S., & Novianti, I. (2017). Efektivitas Atraktan Alami Terhadap Aedes aegypti Pada Perbedaan Warna Perangkap Effectiveness Natural Attractant To Aedes aegypti Into Color Differences Trapping. Jurnal Biologi Dan Pembelajaran Biologi, 2(2), 23–32. https://doi.org/10.32528/bioma.v2i2.819

Ong, J., Chong, CS., Yap, G., Lee, C., Razak, M.A.A., Chiang, S., Ng, LC.,. (2020). Gravitrap deployment for adult Aedes aegypti surveillance and its impact on dengue cases. Ploos Neglected Tropical Disease,14(8), e0008528. https://doi.org/10.1371/journal.pntd.0008528

Kemenkes. (2023). Info Kasus DBD 2023 Minggu ke 19. https://p2pm.kemkes.go.id/publikasi/infografis/info-kasus-dbd-2023-minggu-ke-19

Kemenkes. (2023). Pemberantasan Sarang Nyamuk dengan 3M pluus. https://ayosehat.kemkes.go.id/pemberantasan-sarang-nyamuk-dengan-3m-plus#:~:text= Langkah ini biasa disebut dengan, membawa virus DBD pada manusia

Khairunisa ummi, N. E. wahyuningsih, & Hapsari. (2017). Kepadatan Jentik Nyamuk Aedes sp. (House Index) Sebagai Indikator Surveilans Vektor Demam Berdarah Dengue Di Kota Semarang. Jurnal Kesehatan Masyarakat (e-Journal), 5(5), 906–910. http://ejournal3.undip.ac.id/index.php/jkm

Kurniawati, R. D., Sutriyawan, A., & Rahmawati, S. R. (2020). Analisis Pengetahuan dan Motivasi Pemakaian Ovitrap Sebagai Upaya Pengendalian Jentik Nyamuk Aedes Aegepty. Jurnal Ilmu Kesehatan Masyarakat, 9(04), 248–253. https://doi.org/10.33221/jikm.v9i04.813

Leandro, A., & Maciel-de-Freitas, R. (2024). Development of an integrated surveillance system to improve preparedness for arbovirus outbreaks in a dengue endemic setting: Descriptive study. JMIR Public Health and Surveillance, 10, e62759. https://doi.org/10.2196/62759

Liu, Q.-M., Gong, Z.-Y., & Wang, Z. (2022). A review of the surveillance techniques for Aedes albopictus. The American Journal of Tropical Medicine and Hygiene, 108(2), 245-251. https://doi.org/10.4269/ajtmh.20-0781

Machange, J. J., Maasayi, M. S., Mundi, J., Moore, J., Muganga, J. B., Odufuwa, O. G., Moore, S. J., & Tenywa, F. C. (2024). Comparison of the trapping efficacy of locally modified Gravid Aedes Trap and Autocidal Gravid Ovitrap for the monitoring and surveillance of Aedes aegypti mosquitoes in Tanzania. Insects, 15(6), 401. https://doi.org/10.3390/insects1506040

Nurpadila, I. (2022). Rekayasa Alat Ovitrap Yang Berfungsi Sebagai Perangkap Nyamuk Dalam Upaya

Penurunan Penyebaran DBD Karya Tulis Ilmiah. Tasik: Universitas Bakti Tunas Husada.

Paz-Bailey, G., Adams, L. E., Deen, J., Anderson, K. B., & Katzelnick, L. C. (2024). Dengue. The Lancet, 403(10427), 667-682. https://doi.org/10.1016/S0140-6736(23)02576-X

Rahayu, A., Mutiara, H., Rosa, E., Kedokteran, F., Lampung, U., Ilmu, B., Anatomi, P., Kedokteran, F., Lampung, U., Parasitologi, B., Kedokteran, F., & Lampung, U. (2019). Pengaruh Berbagai Konsentrasi Air Rendaman Jerami Terhadap Jumlah Telur Nyamuk Di Desa Sukajaya Punduh Kecamatan Marga Punduh Kabupaten Pesawaran Provinsi Lampung. Medula, 9, 148–153.

Ridha, M. R., Hairani, B., Melyanie, G., Sari, W., Giri, R., Fadilly, A., & Rosanji, A. (2020). Effectivity of Rice Straw Immersion (Oryza sativa L) and Temephos as Attractant to Lethal Ovitrap Aedes aegypti (in Indonesian Language). Jurnal Ekologi Kesehatan, 19, 112–118. http://repository.lppm.unila.ac.id/16814/1/JeramiNyamuk-Anita%2CHn.pdf

Ritchie, S. A., Buhagiar, T. S., Townsend, M., Hoffmann, A., Van Den Hurk, A. F., McMahon, J. L., & Eiras, A. E. (2014). Field validation of the Gravid Aedes Trap (GAT) for collection of Aedes aegypti (Diptera: Culicidae). Journal of Medical Entomology, 51(1), 210-219. https://doi.org/10.1603/ME13105

Setryawan, A. (2020). Epidemiological Determinants Dengue Hemorrhagic Fever (DHF) In Urban Area: A Retrospective Study Agung. JNPH, 8(2), 1–9. https://doi.org/10.37676/jnph.v8i2.1173

Suparyati. (2020). Uji Daya Bunuh Abate Berdasarkan Dosis Dan Waktu Terhadap Kematian Larva NyamuK Aedes sp dan Culex sp. Pena Jurnal Ilmu Pengetahuan dan Teknologi, 34(2), 1–9. https://doi.org/10.31941/jurnalpena.v34i2.1193

World Health Organization. (2025). Dengue and severe dengue. https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue