ANGKA LEMPENG TOTAL, MOST PROBABLE NUMBER, DAN IDENTIFIKASI BAKTERI COLIFORM PADA SUSU SAPI SEGAR DI KABUPATEN BANYUWANGI

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Published: Apr 4, 2023
DOI: https://doi.org/10.53699/joimedlabs.v4i1.114
Keywords:
Angka Lempeng Total, Coliform, Most Probable Number, Susu Sapi Segar Coliform, Most Probable Number, Raw Cow Milk, Total Plate Count

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Eka Yunita Wulandari
Teknologi Laboratorium Medik, STIKES Banyuwangi, Jawa Timur, Indonesia

Abstract

Coliform bacteria are often found in Fresh cows milk due to their contamination to the environment, cattle feed and drinking, unsterilized equipment, and unaseptic milking processes. Banyuwangi is an area with several cattle farms and common consumption of raw cow milk. This study aimed to determine the total plate count (TPC), most probable number (MPN), and identification of coliform bacteria in raw cow milk in Kabupaten Banyuwangi. Analytical descriptive research was conducted on 30 samples of raw cow milk from 5 cattle farms in Kabupaten Banyuwangi between April and June 2021. The results showed that 7 (23.3%) samples in this study met the TPC requirement but 23 (76.7%) samples exceeded the TPC by SNI. In addition, 10 (33.3%) samples met the MPN requirements but 20 (66.7%) samples exceeded the MPN by SNI. It was concluded that most of fresh cows milk in Kabupaten Banyuwangi have not been eligible to consume due to high TPC, MPN and coliform contamination. Common coliform bacteria found in Fresh cows milk in Kabupaten Banyuwangi were Escherichia coli, Enterobacter sp., and Klebsiella sp., respectively. Further study was needed to identify coliform species and analyze other parameters of fresh cows milk in Kabupaten Banyuwangi according to SNI.

Article Details

How to Cite
Wulandari, E. Y. (2023). ANGKA LEMPENG TOTAL, MOST PROBABLE NUMBER, DAN IDENTIFIKASI BAKTERI COLIFORM PADA SUSU SAPI SEGAR DI KABUPATEN BANYUWANGI. Journal of Indonesian Medical Laboratory and Science (JoIMedLabS), 4(1), 45-58. https://doi.org/10.53699/joimedlabs.v4i1.114
Issue
Vol. 4 No. 1: April 2023
Section
Artikel

References

Abebe, E., Gugsa, G., & Ahmed, M. (2020). Review on Major Food-Borne Zoonotic Bacterial Pathogens. Journal of Tropical Medicine, 2020, 1–19. https://doi.org/10.1155/2020/4674235.

Adetunji, S. A., Ramirez, G., Ficht, A. R., Perez, L., Foster, M. J., & Arenas-Gamboa, A. M. (2020). Building the Evidence Base for the Prevention of Raw Milk-Acquired Brucellosis: A Systematic Review. Frontiers in Public Health, 8, 76. https://doi.org/10.3389/FPUBH.2020.00076.

Aria Suzanni, M., Iqbal, M., & Irhamni. (2021). Uji Cemaran Mikroba Pada Susu Kedelai Produksi Rumah Tangga Yang Beredar Di Banda Aceh Dengan Metode TPC. Jurnal Pendidikan Dan Pengabdian Vokasi, 2(1), 111–114.

Asfidoajani, F. A., & Sichani, M. M. (2018). Comparison of Coliform Contamination and Endotoxin Levels in Raw Cow’s Milk. Zahedan Journal of Research in Medical Sciences, 20(5). https://doi.org/10.5812/zjrms.59333.

Badan Standarisasi Nasional. (2011). Susu Segar-Bagian 1?: Sapi (Badan Standarisasi Nasional, Ed.).

Davin-Regli, A., Lavigne, J.-P., & Pagès, J.-M. (2019). Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance. Clinical Microbiology Reviews, 32(4). https://doi.org/10.1128/CMR.00002-19.

Grace, D., Wu, F., & Havelaar, A. H. (2020). MILK Symposium review: Foodborne diseases from milk and milk products in developing countries—Review of causes and health and economic implications *. Journal of Dairy Science, 103(11), 9715–9729. https://doi.org/10.3168/JDS.2020-18323.

Haskito, A. E. P., Sari, C., & Dameanti, F. N. A. E. P. (2019). Gambaran pengetahuan siswa SMAN 8 Malang tentang foodborne disease. ARSHI Veterinary Letters, 3(1), 15–16. https://doi.org/10.29244/avl.3.1.15-16.

Kemenkes. (2021). Profil Kesehatan Indonesia Tahun 2020. Kementrian Kesehatan Republik Indonesia.

Kumalasari, E., Prihandiwati, E., & Rhodiana. (2018). Analisis Kuantitatif Bakteri Coliform Pada Depot Air Minum Isi Ulang Yang Berada Di Wilayah Kayutangi Kota Banjarmasin. Jurnal Ilmiah Ibnu Sina, 3(1), 134–144.

Kusumaningsih, A., & Ariyanti, T. (2013). Cemaran Bakteri Patogenik pada Susu Sapi Segar dan Resistensinya terhadap Antibiotika. Jurnal Ilmu-Ilmu Hayati, Vol. 12(1)(April), Pp. 9-17.

Massé, J., Dufour, S., & Archambault, M. (2020). Characterization of Klebsiella isolates obtained from clinical mastitis cases in dairy cattle. Journal of Dairy Science, 103(4), 3392–3400. https://doi.org/10.3168/jds.2019-17324.

Mokadem, E., Leboudy, A., & Amer, A. (2020). Occurrence of Enterobacteriacae in Dairy Farm Milk. Alexandria Journal of Veterinary Sciences, 64(2), 66. https://doi.org/10.5455/ajvs.254389.

Muthaz, B. D. A., Karimuna, S. R., & Ardiansyah, R. T. (2017). Studi Kualitas Air Minum Di Desa Balo Kecamatan Kabaena Timur Kabupaten Bombana Tahun 2016. Jurnal Ilmiah Mahasiswa Kesehatan Masyarakat, 2(5), 1–9.

Nero, L. A., & De Carvalho, A. F. (2019). Challenges for Production and Consumption of Raw Milk and Raw Milk Products. In L. A. Nero & A. F. De Carvalho (Eds.), Raw Milk: Balance Between Hazards and Benefits (pp. 351–362). Academic Press. https://doi.org/10.1016/B978-0-12-810530-6.00016-X.

Nurmawati, S., Prodjosoewojo, S., Chairunnisa, N. H., Djauhari, H., & Alisjahbana, B. (2019). Faktor Risiko Penyebab Foodborne Disease pada Siswa SD. Jurnal Sistem Kesehatan, 4(4), 180–184.

Pradika, A. Y., Chusniati, S., Purnama, M. T. E., Effendi, M. H., Yudhana, A., & Wibawati, P. A. (2019). Uji Total Escherichia coli pada Susu Sapi Segar di Koperasi Peternak Sapi Perah (KPSP) Karyo Ngremboko Kecamatan Purwoharjo Kabupaten Banyuwangi. Jurnal Medik Veteriner, 2(1), 1. https://doi.org/10.20473/jmv.vol2.iss1.2019.1-6.

Pramesthi, R., & Hari, T. (2015). Total bakteri dan ph susu segar sapi perah friesian holstein di unit pelaksana teknis daerah dan pembibitan ternak unggul mulyorejo tengaran- semarang. Animal Agriculture Journal, 4(1), 69–74.

Salman, A., & Hamad, I. (2011). Enumeration and identification of coliform bacteria from raw milk in Khartoum State, Sudan. Journal of Cell and Animal Biology, 5(7), 121–128. https://doi.org/10.3923/ijds.2011.66.71.

Santoso, L., Rukmi, I., & Lestari, O. (2012). Jumlah Total Bakteri Dan Coliform Dalam Air Susu Sapi Segar Pada Pedagang Pengecer di Kota Semarang. Jurnal Kesehatan Masyarakat, 1, 402–412.

Saputri, E. T., & Efendy, M. (2020). Kepadatan Bakteri Coliform Sebagai Indikator Pencemaran Biologis Di Perairan Pesisir Sepuluh Kabupaten Bangkalan. Juvenil:Jurnal Ilmiah Kelautan Dan Perikanan, 1(2), 243–249. https://doi.org/10.21107/juvenil.v1i2.7579.

Sari, D. P., Rahmawati, & Rusmiyanto, E. (2019). Deteksi dan Identifikasi Genera Bakteri Coliform Hasil Isolasi dari Minuman Lidah Buaya. Jurnal Labora Medika, 3(1), 29–35.

Sengupta, C., & Saha, R. (2013). Understanding coliforms - a short review. International Journal of Advanced Research, 1(4), 16–25.

Suhailah, L., & Santoso, T. (2018). Analisa Cemaran Bakteri Coliform Pada Susu Sapi Murni dengan Variasi Lama Penyimpanan Dalam Suhu Freezer dan Suhu Kulkas di Desa Wilayut Sukodono Sidoarjo. Jurnal Sains, 8(5), 44–49.

Sutton, S. (2011). Accuracy of Plate Counts. Journal of Validation Technology, 17(3), 42–46.

Todd, E. (2020). Food-Borne Disease Prevention and Risk Assessment. International Journal of Environmental Research and Public Health, 17(14), 1–13. https://doi.org/10.3390/IJERPH17145129

Tominaga, T., & Ishii, M. (2020). Detection of microorganisms with lateral flow test strips. Methods in Microbiology, 47, 351–394. https://doi.org/10.1016/BS.MIM.2019.11.007.

Yang, Y., Peng, Y., Jiang, J., Gong, Z., Zhu, H., Wang, K., Zhou, Q., Tian, Y., Qin, A., Yang, Z., & Shang, S. (2021). Isolation and characterization of multidrug-resistant Klebsiella pneumoniae from raw cow milk in Jiangsu and Shandong provinces, China. Transboundary and Emerging Diseases, 68(3), 1033–1039. https://doi.org/10.1111/tbed.13787.

Younis, G., Awad, A., & Ashraf, N. (2017). Molecular and Phenotypic Characterization of Antimicrobial Resistance in Gram Negative Bacteria Recovered from Subclinical Mastitis. Advances in Animal and Veterinary Sciences, 5(5), 196–204. https://doi.org/10.17582/journal.aavs/2017/5.5.196.204.