Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)

Identification of eggs of soil transmitted helminthes (STH) intestinal nemathodes in children by flotation method in Tengket village, Arosbaya district, Bangkalan regency

2023-09-27T04:04:54+00:00

Background: Worm infections can occur in humans throughout the world regardless of age and attack children's health. This statement encouraged researchers to do this in Tengket Village, where most guardians ignore their children's PBHS and latrines in rivers around residents' houses cause worm infections. More than 1.5billion people, or 24% of the world's population, are infected with soil-borne worms. That are transmitted through the soil are also called nematodes of the STH class. Objectives: The study aimed to identify STH intestinal nematode eggs in children in Tengket Village, Arosbaya, Bangkalan. Materials and Methods: The method used is flotation using NaCl reagent with the principle of different specific gravity so the parasites in the feces will be floated on the surface. The floatation method is able to produce a clean field of view and an easy procedure, thereby minimizing false positives and false negatives. Results: The research was carried out in February 2023 and the results obtained were that 9 out of 30 children's feces samples tested positive for worms. The positive results in the samples could be due to river latrines still being used and the spread occurring due to lack of attention to PHBS. Conclusions: Based on the research results, the percentage of worms has increased.

Examination for worms soil transmitted helminths with the influence of PHBS on children in Karadenan village, Bogor regency

2023-09-27T03:37:10+00:00

Background: Worms are a parasitic infection in the form of worms entering the human body. One of the factors that causes worms is personal hygiene. The aim of the research is to study the relationship between risk factors and the effects of worms on children's health. Objectives: One place that has a risk of worms is the village of Keradenan, Bogor district, because the condition of the village is slum. Materials and Methods: This type of research is analytical with a cross sectional research design. The sample in this study was 96 people who met the inclusion and exclusion criteria using a purposive sampling technique. Data collection was carried out by filling out questionnaires and checking for worms (soil transmitted helminths) using native methods. Data processing uses statistical software with the Chi square test. Results: The incidence of worms was 13.54% of the 96 samples of the type of Soil Transmitted Helminthes worm that infects children, namely Ascaris Lumbricoides. Conclusions: Based on the results of the research, there is an influence on clean and healthy living behavior on the children of the village of Keradenan, the result of worm examinations is that the number of worms has decreased.

Determination of uncertainty measurement using top-down approach and its correlation towards Total Error (TE) on hematology parameter

2023-11-30T00:40:44+00:00

Background: Uncertainty Measurement (UM) is defined as a parameter associated with a measurement result that characterizes a dispersion of values that can reasonably be associated with a quantity. ISO 15189 clause 5.5.1.4. states that the laboratory shall determine measurement uncertainty for each measurement procedure in the examination phase used to report measured quantity values on patients samples. Objectives: According to Westgard, there is debate about difference between the concepts of Uncertainty and Total Error (TE) so this study was conducted which aims to determine the uncertainty value of hematological parameters and determine the correlation of Uncertainty and TE. Materials and Methods: The material in this research are data of Internal Quality Control (IQC), External Quality Control (EQC), and Uncertainty data from the Sysmex XN-1000 calibrator. Results: Through the results the average uncertainty value of Sysmex XN-1000 2022 for leukocyte with low, medium and high levels, are ±19.05%, ±18.07 %, and ±15.94%, while for erythrocyte are ±4.46%, 4.10%, 4.16%, for hemoglobin are ±5.63, ±5.07, ±5.01, for hematocrit are ±8.99%, ±8.19%, ±8.19%, and for platelet are ±79.23%, ±62.23%, ±58.29%. Conclusions: Based on the uncertainty and TE calculated permonth for each lot number during 2022, the correlation was obtained between the Uncertainty and TE among leukocyte and hematocrit were stated to have a weak correlation, for hemoglobin and platelet it was stated quite correlated and erythrocytes are stated to be strongly correlated.

Identification of Ascaris lumbricoides Egg in cauliflower (Brassica oleracea var. botrytis) by sedimentation method

2023-10-06T08:14:59+00:00

Background: Ascaris lumbricoides is the causative agent of a helminthic diseases known as ascariasis. According to the World Health Organization (WHO), helminthic diseases constitute more than half of the health issues in developing countries. It is caused by poor sanitation and a tropical climate that supports the development of worms. Transmission of worm eggs to humans can be through vegetables that grow near the ground, one of them is cauliflower. Objectives: The study aimed to identify the presence of Ascaris lumbricoides eggs in cauliflower sold at Krembung Market, Sidoarjo Regency. Materials and Methods: The method used is sedimentation using 0,2% NaOH reagent with the principle of centrifugal force which results in the separation of worm eggs and supernatant. This method can precipitate eggs without damaging them, making it easier to identify the type of worm eggs. Results: The research was carried out in April 2023 and the results showed that from the 12 samples sold on mats, there were 2 samples (17%) contaminated with infertile Ascaris lumbricoides eggs. Meanwhile, from 12 samples sold on table, no Ascaris lumbricoides eggs (0%) were found. Conclusions: Based on the research results, Ascaris lumbricoides eggs were found in 2 out of 12 samples of cauliflower sold on mats.

Analysis of total cholesterol levels in public infrastructure and facilities officers (petugas PSU) in Kelurahan Rawa Buaya Jakarta Barat

2023-09-28T13:50:00+00:00

Background: The basic need for food must be fulfilled by humans as a fulfillment of energy sources in carrying out activities. Consumption of fat macromolecules has the highest energy compared to other macromolecules. However, excessive consumption of fat can also increase cholesterol in the blood. Public Infrastructure and Facilities Officers (PPSU) do a lot of outdoor work so it is possible to have unhealthy eating habits such as frequently consuming fatty foods. Objectives: The purpose of this study was to determine qualitatively the distribution of total cholesterol levels in PPSU in Rawa Buaya Village. This research is descriptive-analytic. Materials and Methods: The research sample was obtained using a purposive sampling technique with inclusion and exclusion criteria involving 67 respondents. Results: The results showed that the majority of respondents were male, with 65 respondents (97%). From the results of the age group category, 24 people (35.8%) were dominated by respondents aged 31-40 years. Conclusions: The overall average cholesterol level of respondents is 201 mg/dL. If distributed by gender, female respondents have an average total cholesterol level of 237 mg/dl and men have an average total cholesterol level of 200 mg/dL.

The relationship of leukocyte number and plattle number in patients with dengue fever (DHF) at Puskesmas Piyungan

2024-01-02T08:52:32+00:00

Background: Dengue Hemorrhargic Fever (DHF) is an infectious disease caused by the dengue virus. Dengue fever is transmitted by Aedes aegypti and Aedes albopictus mosquitoes. In dengue fever sufferers, when a complete blood test is performed, the most common result is a decrease in the number of leukocytes and the number of platelets. Objectives: This study aims to determine the relationship between the number of leukocytes and platelets in DHF patients at Puskesmas (primary health center) Piyungan. Materials and Methods: The type of research used was analytical observational with a cross sectional research design and the data were collected using total sampling techniques. Data was obtained from medical records with a sample size of 26 patients. The relationship between the number of leukocytes and the number of platelets in dengue fever sufferers was tested using the Spearman’s correlation test. Results: The result of analysis using the Spearman’s correlation test did not show a significant relationship between the number of leukocytes and the number of platelets in dengue fever patients (p = 0.0774), and the level of relationship was very weak with a positive relationship direction (r = 0.059). Conclusions: there was no significant relationship between the number of leukocytes and the number of platelets in dengue fever patients at Puskesmas Piyungan. A decrease in the number of leukocytes and platelets is a common condition that is often found in dengue fever patients.

Crystal sediment profile in the urine of public transportation drivers on route 2 in Kupang city

2023-09-28T13:44:33+00:00

Background: Drivers have a profession that requires prolonged sitting, leading to the release of bone calcium into the bloodstream, which can trigger the formation of urinary stones or nephrolithiasis. Urinary stone (nephrolithiasis) is a condition where stones form in the urinary tract. Objectives: The aim of this study was to describe the crystal sediment profile in the urine of public transportation drivers on Route 2 in Kupang City. Materials and Methods: This descriptive study involved 16 public transportation drivers on Route 2 in Kupang City as research subjects. Data were collected through interviews. Results: The results showed the presence of various crystals in the urine sediment, including Calcium Oxalate crystals (25%), Calcium Carbonate crystals (18.75%), Uric Acid crystals (12.5%), and Amorphous crystals (6.25%). Conclusions: Based on the characteristics and habits of the respondents, it was found that frequent urinary retention, drinking less than 1 liter of water per day, and consuming supplemented or carbonated drinks were the three main factors affecting urine sedimentation in public transportation drivers on Route 2 in Kupang City.

Relationship between urinary track infection screening tests and urine culture results in patients with suspect urinary track infection

2023-09-27T03:53:48+00:00

Background: Urinary tract infection (UTI) is a common disease found in the world. Gold standard for the diagnosis of UTI is urine culture, but this examination has the disadvantage of taking longer results. As a screening test, urine dipstick and urine flowcytometry provide more rapid diagnosis of UTI. Objectives: The purpose of this study was to determine the relationship between the results of the dipstick test and UF4000 with urine culture. Materials and Methods: This study used an analytic observational method with a cross sectional approach, with accidental sampling. The samples obtained was 38 samples from patients at the Clinical Laboratory of Prodia Diponegoro and Prodia SDP. Results: The results showed that there was 84.2% concordance between leukocyte esterase results and culture results, and 31.6% concordance between nitrite results and culture results, 97.4 5% concordance between urine flowcytometry results and urine culture results. Conclusions: Based on the Chi square statistical, it was concluded that there were relationship between Urine culture and leukocyte esterase (p-value 0.000), nitrite (p-value 0.004, urine flowcytometri (p-value 0.000). Both of these screening tests can be used to help diagnose UTI, but the gold standard for UTI still uses urine culture result.